Peptide reconstitution calculator.
Enter the milligrams of lyophilized material in the vial and the volume of bacteriostatic water you plan to add. The calculator returns the resulting concentration in mg/mL, the micrograms per syringe unit, and the volume that corresponds to a target amount. For laboratory reference and record-keeping only.
Total lyophilized material stated on the label or COA.
Final volume in the vial after reconstitution.
Optional — used to convert to a draw volume.
- Concentration
- 2.5 mg/mL
- Concentration
- 2,500 mcg/mL
- Per syringe unit
- 25 mcg / unit
- Draw volume
- 0.1 mL
- Draw on syringe
- 10 units
- Draws per vial
- 20
Figures are arithmetic conversions only. Verify every value against your laboratory SOP before recording it. Nothing on this page is a protocol, and no product sold here is for human or animal use.
The formulas
mg/mL = vial amount (mg) ÷ diluent volume (mL)
5 mg in 2 mL = 2.5 mg/mL
mcg/unit = (mg/mL × 1000) ÷ units per mL
2.5 mg/mL on a U-100 syringe = 25 mcg per unit
mL = target amount (mg) ÷ concentration (mg/mL)
250 mcg at 2.5 mg/mL = 0.1 mL
draws = diluent volume (mL) ÷ draw volume (mL)
2 mL ÷ 0.1 mL = 20 draws
Common questions
What is the peptide reconstitution formula?
Concentration (mg/mL) = vial amount in mg ÷ diluent volume in mL. To find a draw volume: volume (mL) = target amount in mg ÷ concentration in mg/mL.
How many units are in 1 mL on an insulin syringe?
A standard U-100 insulin syringe has 100 units per millilitre, so 1 unit equals 0.01 mL. A U-40 syringe has 40 units per millilitre.
Which diluent is used for lyophilized research material?
Bacteriostatic water is the common diluent for laboratory handling because the benzyl alcohol content limits microbial growth in a multi-draw vial. Follow the diluent and storage conditions specified in your laboratory SOP.
Does dissolving the material change the total volume?
For the milligram quantities used in typical laboratory vials the displaced volume is negligible, so the diluent volume is treated as the final volume.
More handling and storage answers are in the FAQ, and every lot's testing record is covered on the process page.